Plasmid editor — ASO

By young-Hwan Jo on AppTrack Ninja.

Vector

Import a SnapGene, GenBank or FASTA file. Every element is matched against the bases rather than trusted from the label, and each one is marked with how it was established: confirmed by exact sequence match, taken from the file's own annotation, or predicted from structure alone. 1. FOUR EDITS Replace the promoter. Add a gene. Remove a gene. Swap a part. Each one produces a finished construct, the Gibson assembly plan, and primers with melting temperatures. Homology arms extend until they are unique, so repeated sequence does not produce an ambiguous junction. Every design is re-assembled in …

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